CLS 513 Advanced Diagnostic Microbiology

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One-step, safe and rapid extraction of Brucella strains by MALDITOF mass spectrometry.

First, you should introduce the paper and describe the methodology. Next, discuss the findings and their meaning.


Brucellosis is a zoonotic infection that can infect domestic livestock worldwide. It remains a risk to these animals and a threat to public health and financial loss.

Brucellosis is a zoonotic illness that can infect humans through contaminated milk products and meat. This makes it imperative to have a fast and accurate method of identifying the disease.

The current methods for identification are expensive and time-consuming. However, MALDITOF base mass spectrumrometry is a promising alternative.

The authors have created a new protocol for protein extraction and a customized reference database.

This database included a list of Brucella strains that are widely spread.

This database was then compared to infected samples from infected animals.

The assay was capable of identifying 99.5 percent of Brucella strains in the genus and 97.9% of Brucella species.

This means that the assays were not affected by microbial isolation in different culture conditions.

This assay is reliable because it does not affect protein stability when stored and sent to comparison databases.

The MALDI-TOF test was unable to determine a phylogenetic tree, meaning that the protein profile is not consistent with the evolution of genes.

This MALDI-TOF method is reliable, fast, and accurate based on the collected data.

These are the questions that were raised after thorough review and analysis of this article:

What steps and factors should be taken to improve their protocol so it can be immediately used in industry and the health sector?

Which measures or elements can be included in the assay protocol to accurately decipher a Dendrogram?

It can take up to 72 hours to ship a sample from a remote location to a laboratory in countries such as Africa.

If the samples are kept for more than 72 hours, would this still make it reliable and effective?

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